In Vitro Antioxidant Study of Methanolic Extracts of the Seed Kernel of Mangifera Indica & Artocarpus Heterophyllus

Abstract

The present study was subjected to investigate antioxidant property of methanolic extracts of the seed kernel of Mangifera indica & Artocarpus heterophyllus. For antioxidant properties- Total Phenols, Total flavonoids and DPPH free radical scavenging capacity of the methanolic extracts of the sample were assessed. Total phenolic constituents determination of the plant extract were performed employing the literature methods involving Folin-Ciocalteu reagent and gallic acid as standard .Moreover, total phenolic concentration equivalents to Gallic acid for Mangifera indica was found 433 ± 0.71 mg/gm of extract and for Artocarpus heterophyllus, it was 100.75 ± 10.253 mg/gm of extract which is correlated with antioxidant activity. The Total Flavonoid contents of the test sample were calculated using the standard curve of Quercetin, the value of Mangifera indica was 129 ± 1.414 mg QUE/gm of extract and the quantity of Artocarpus heterophyllus was 52.25 ± 1.06 mg QUE/gm of extract. DPPH free radical scavenging effect of the extract was assessed. The DPPH result of the sample was compared with standard ascorbic acid. IC₅₀ values of Mangifera indica and Artocarpus heterophyllus were 7.3μg/ml and 335.77μg/ml respectively which was comparable to that of the standard ascorbic acid (15.11µg/ml). So, the seed kernel of Mangifera indica can be regarded as suitable agent of natural antioxidant having high phenolic and flavonoid contents.